| 產品名稱 |
K:Molv NIH/3T3 |
| 商品貨號 |
B164897 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells are infected with the Kirsten strain of Moloney murine sarcoma virus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
sarcoma |
| Age |
embryo |
| Strain |
NIH/Swiss |
| Storage Conditions |
liquid nitrogen vapor phase |
| Comments |
The cells are infected with the Kirsten strain of Moloney murine sarcoma virus.
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:25 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
