| 產品名稱 |
KG-1 |
| 商品貨號 |
B164908 |
| Organism |
Homo sapiens, human |
| Tissue |
bone marrow |
| Cell Type |
Macrophage |
| Product Format |
frozen |
| Morphology |
myeloblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute myelogenous leukemia |
| Age |
59 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations. |
| Derivation |
The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. |
| Clinical Data |
59 years
Caucasian
male |
| Antigen Expression |
HLA A30, A31, B35, Cw4 |
| Genes Expressed |
HLA DR |
| Cellular Products |
HLA DR |
| Comments |
KG-1 myeloblasts can differentiate into macrophages in the presence of phorbol esters.
They show a good response to colony stimulating factor (CSF).
The line is EBNA negative (EBNA-). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL. Do not allow to exceed 2 x 106 cells/mL.
Medium Renewal: Twice per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 |
| Isoenzymes |
AK-1, 0 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 0 |
| Name of Depositor |
DW Golde |
| Deposited As |
Homo sapiens |
| References |
Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765
Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689
Hester JP et al. Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel. Blood 54(1): 254-268 1979. PubMed: 444670
|
| Cross References |
Nucleotide (GenBank) :
AF146432
Homo sapiens cell-line KG-1a sodium/hydrogen exchanger isoform 1 mRNA, complete cds.
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