| Comments |
The antibody reacts with denatured beta chains of the b, d, p, q, j and bu haplotypes.
It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface.
It does not react with molecules of the k, f, and s haplotypes.
The reactive determinant is most likely involves residues 153 through 155.
Depositor recommends using low IgG serum. ATCC uses ultra-low IgG fetal bovine serum (Invitrogen catalog # 16250). |
| References |
LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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