| 產品名稱 | KLN 205 |
|---|---|
| 商品貨號 | B164918 |
| Organism | Mus musculus, mouse |
| Tissue | lung |
| Product Format | frozen |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | squamous cell carcinoma |
| Strain | DBA/2 |
| Storage Conditions | lquid nitrogen vapor phase |
| Images |  |
| Tumorigenic | Yes |
| Effects | Yes, in DBA/2 and BDF1 mice |
| Comments | KLN 205 cells form metastatic lesions in lungs after inoculation into mice. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: lquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | T Kaneko |
| Deposited As | Mus musculus |
| References | Kaneko T, LePage GA. Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo. Cancer Res. 38: 2084-2090, 1978. PubMed: 418873 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
