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KMA

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編號:B164923

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產品名稱	KMA
商品貨號	B164923
Organism	Homo sapiens, human
Tissue	spleen
Product Format	frozen
Morphology	monocyte/macrophage
Culture Properties	suspension, multicell aggregates, Single cells are usually nonadherent but may become adherent.
Biosafety Level	2 [Cells contain Epstein-Barr Viral DNA sequences] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	The KMA cell line was established from cryopreserved human spleen cells.
Complete Growth Medium	Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 10⁵ viable cells/mL. Maintain cell density between 2 x 10⁵and 2 x 10⁶ viable cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 12,13 D5S818: 12,13 D7S820: 11,12 THO1: 6,9 TPOX: 8,9 vWA: 17
Name of Depositor	E.I. du Pont de Nemours & Co.
Deposited As	Homo sapiens
U.S. Patent Number	5,447,861
References	Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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