| 產品名稱 |
L368 |
| 商品貨號 |
B164944 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin) |
| Comments |
Animals were immunized with the RPMI 8866 human B cell line. Spleen cells were fused with NS-1 myeloma cells. The antibody does not cross-react with mouse, rat, cat or monkey beta-2-microglobulin. The antibody is not cytotoxic. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
LA Lampson |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lampson LA, et al. Striking paucity of HLA-A, B, C and beta2-microglobulin on human neuroblastoma cell lines. J. Immunol. 130: 2471-2478, 1983. PubMed: 6187860
|
