| 產品名稱 |
LC-540 |
| 商品貨號 |
B164965 |
| Organism |
Rattus norvegicus, rat |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Gender |
male |
| Strain |
Fischer |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 42; range = 40 to 43.
Approximately 21% of the cells examined were aneuploids. No other structural abnormalities were noted. |
| Clinical Data |
male |
| Tumorigenic |
Yes |
| Effects |
Yes, in rats |
| Virus Susceptibility |
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
|
| Virus Resistance |
poliovirus 1 |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor |
A Steinberger |
| Deposited As |
Rattus sp. |
| Year of Origin |
October, 1969 |
| References |
Jacobs BB, Huseby RA. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J. Natl. Cancer Inst. 41: 1141-1153, 1968. PubMed: 5688092
|
