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The tissue donor suffered from an atypical form of Marfan syndrome.

The patient presented with no eye findings, normal height and severe joint laxity.

female

The tissue donor suffered from an atypical form of Marfan syndrome.

The patient presented with no eye findings, normal height and severe joint laxity.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.