| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| References |
American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956.
Hull RN. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor cells on glass. Science 117:223, 1953. Pubmed : 13038481
Hull RN, Cherry WR, Johnson IS. The adaptation and maintenance of mammalian cells to continuous growth in tissue culture. Anat Rec 124: 490–499,1956.
Earle WR. A study of the Walker rat mammary carcinoma 256, in vivo and in vitro. Am J Cancer 24:566-612, 1935.
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