| 產品名稱 |
LP4.4 |
| 商品貨號 |
B165005 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with most human IgM, lambda and some human IgM, kappa molecules. It also reacts with mu - surrogate light chain complexes, and provides a marker for pre-B and early B cell differentiation. |
| Derivation |
Lymph node cells were fused with P3X63Ag8.653 myeloma cells. It does not react with isolated mu, lambda or kappa chains. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human IgM, lambda |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human IgM, lambda |
| Comments |
Animals were immunized with a human IgM, kappa paraprotein (LP) which reacted with intermediate filaments. Lymph node cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with most human IgM, lambda and some human IgM, kappa molecules. It appears to react with a conformational determinant expressed preferentially on IgM, lambda complexes. It also reacts with mu - surrogate light chain complexes, and provides a marker for pre-B and early B cell differentiation. It does not react with isolated mu, lambda or kappa chains. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 50 uM 2-mercaptoethanol, 90%; fetal
bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b; kappa light chain |
| Name of Depositor |
H Kubagawa, JF Kearney |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ohno T, et al. A mouse monoclonal antibody reactive preferentially with human IgM lambda. Hybridoma 9: 473-480, 1990. PubMed: 1701760
|
