| 產品名稱 |
LS 102.9 |
| 商品貨號 |
B165006 |
| Organism |
Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). |
| Comments |
The LS 102.9 bears surface I-A d,s and I-E d molecules and can present antigen to appropriate I region restricted T cell hybridomas. The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below) with 50 uM
2-mercaptoethanol, 90%; fetal bovine serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml Maintain pH below 7.2. |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
P Marrack, J Kappler |
| Deposited As |
mouse (B cell); mouse (lymphoma) |
| References |
Kappler J, et al. Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413
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