| 產品名稱 |
LS-136 |
| 商品貨號 |
B165011 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein. The antibody is specific for the C1 domain. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified B1-8 monoclonal antibody. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain |
| Comments |
Animals were immunized with purified B1-8 monoclonal antibody. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein. The antibody is specific for the C1 domain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
K Rajewsky |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Reth M, et al. Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl) acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004-1013, 1979. PubMed: 398773
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