LTR228 [LTR 228-TG, derivative of WIL-2NS (ATCC CRL-8155)] (ATCC® HB-8502™)
商品貨號
B165016
Organism
Homo sapiens, human
Tissue
spleen
Cell Type
B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
hereditary spherocytosis
Age
5 years
Gender
male
Ethnicity
Caucasian
Applications
The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.
The cells have a high cloning efficiency in soft agar and by limiting dilution.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 0.03% L-glutamine and 0.02 mM 6-thioguanine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
