| 產品名稱 |
LYK-12 |
| 商品貨號 |
B165019 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: S344NCR (B cell); BALB/c (myeloma) |
| Applications |
Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. The antibody is specific for a determinant that is apparently dependent upon posttranslational modification of CD44. The specific exon coding for the determinant is not known. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a mouse CD44 determinant dependent upon posttranslational modification (specific exon unknown) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a mouse CD44 determinant dependent upon posttranslational modification (specific exon unknown) |
| Comments |
Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody is specific for a determinant that is apparently dependent upon posttranslational modification of CD44. The specific exon coding for the determinant is not known. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with:
0.05 mM 2-mercaptoethanol
1% Origen Hybridoma Cloning Factor
10% fetal bovine serum
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
KS Hathcock, Y Yamashita |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314
|
