| 產品名稱 |
Lym-1 |
| 商品貨號 |
B165025 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody stains the surfaces of normal human B cells and derived tumors, but does not react with the surfaces of solid tumors (some cytoplasmic staining is seen with some tumors. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. The antibody stains the surfaces of normal human B cells and derived tumors, but does not react with the surfaces of solid tumors (some cytoplasmic staining is seen with some tumors. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against normal human B cells and derived malignancies |
| Cellular Products |
immunoglobulin; monoclonal antibody; against normal human B cells and derived malignancies |
| Comments |
Animals were immunized with preparations of nuclei from Raji cells. Spleen cells were fused with NS-1 myeloma cells. The antibody stains the surfaces of normal human B cells and derived tumors, but does not react with the surfaces of solid tumors (some cytoplasmic staining is seen with some tumors. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
Northwestern University |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Epstein AL. Murine hybridoma Lym-1 and diagnostic antibody produced thereby. US Patent 4,724,213 dated Feb 9 1988
|
