| 產品名稱 |
Lym-2 |
| 商品貨號 |
B165026 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells |
| Comments |
Animals were immunized with CLL cells. Spleen cells were fused with NS-1 myeloma cells. The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
Northwestern University |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Epstein AL. Murine hybridoma Lym-2 and diagnostic antibody produced thereby. US Patent 4,724,212 dated Feb 9 1988
|
