| 產品名稱 |
M/K-1.9 |
| 商品貨號 |
B165028 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against murine VCAM-1 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against murine VCAM-1 |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in CB.17.SCID mice |
| Comments |
Animals were immunized with stromal cells from mouse bone marrow. Spleen cells were fused with Sp2/0 myeloma cells. The antibody blocks adhesion of lymphocyte precursors to bone marrow stromal cells. The antibody recognizes an antigen believed to be the mouse equivalent of human VCAM-1 that is expressed on bone marrow stromal cells and on inflamed endothelial cells (see also M/K-2.7, ATCC CRL-1909). |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
PW Kincade |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Miyake K, et al. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J. Cell Biol. 114: 557-565, 1991. PubMed: 1713592
Miyake K, et al. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J. Exp. Med. 173: 599-607, 1991. PubMed: 1997648
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