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M1/70.15.11.5.HL

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編號:B165038

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產品名稱	M1/70.15.11.5.HL
商品貨號	B165038
Organism	Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue	spleen
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody precipitates two polypeptides of 190000 and 105000 daltons, binds to human monocytes, polymorphonuclear leukocytes and a small population of lymphocytes. Tested and found negative for ectromelia virus (mousepox).
Storage Conditions	liquid nitrogen vapor phase
Derivation	Spleen cells were fused with NS-1 myeloma cells. The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Genes Expressed	immunoglobulin; monoclonal antibody; against mouse macrophage, granulocyte (Mac-1, alpha chain)
Cellular Products	immunoglobulin; monoclonal antibody; against mouse macrophage, granulocyte (Mac-1, alpha chain)
Comments	Animals were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells. Mac-1 is a mouse macrophage differentiation associated with type three complement receptor (CR3). The antigen is expressed in large amounts on thioglycollate induced peritoneal exudate macrophages and in lesser quantities on neutrophilic granulocytes, blood monocytes. 8% of spleen cells, 44% of bone marrow cells and less than 0.3% of thymus cells react with the antibody. The antibody precipitates two polypeptides of 190000 and 105000 daltons, binds to human monocytes, polymorphonuclear leukocytes and a small population of lymphocytes. The antibody is capable of both natural killing and antibody dependent cellular cytotoxicity. Tested and found negative for ectromelia virus (mousepox). The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37.0°C
Isotype	rat IgG2b
Name of Depositor	TA Springer
Deposited As	rat (B cell); mouse (myeloma)
References	Springer T, et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539-551, 1978. PubMed: 81133 Springer T, et al. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur. J. Immunol. 9: 301-306, 1979. PubMed: 89034 Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058 Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226 Springer TACell -surface differentiation in the mouse: Characterization of Jumping and Lineage antigens using xenogeneic rat monoclonal antibodiesIn: Springer TAMonoclonal AntibodiesNew YorkPlenum Presspp. 185-217, 1980 Zhang L, Plow EF. Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418 Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913
Cross References	Nucleotide (GenBank) : U12763 Mus musculus OX40 ligand (ox40l) mRNA, complete cds. Nucleotide (GenBank) : NM_009452 Mus musculus tumor necrosis factor (ligand) superfamily, member 4 (Tnfsf4), mRNA.

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