| 產品名稱 |
M3/84.6.34 |
| 商品貨號 |
B165051 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Genes Expressed |
Tested and found negative for ectromelia virus (mousepox). immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein),Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage. |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein) |
| Comments |
Animals were immunized with detergent solubilized mouse(C57BL/6) macrophage membrane which had been depleted of previously identified antigens with monoclonal immunoadsorbants. Like Mac-2, the Mac-3 antigen is not expressed on bone marrow cells (Mac-1 is expressed on bone marrow cells). Also like Mac-2, Mac-3 appears to be expressed on their monocytic line of differentiation at a stage after divergence from the granulocytic series. Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes. Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Adherent cells can be dislodged by scraping and cultures established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
rat IgG1 kappa |
| Name of Depositor |
TA Springer |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058
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