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M5/114.15.2

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編號:B165055

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產品名稱	M5/114.15.2
商品貨號	B165055
Organism	Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue	spleen
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Lymphoma
Strain	(BN x Lewis)F1
Storage Conditions	liquid nitrogen vapor phase
Derivation	Rats were immunized with a secondary mixed lymphocyte culture (C57BL/6 mouse anti irradiated BN rat lymphoma). Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against I-A b, I-A d, I-A q, I-E d and I-E k
Cellular Products	immunoglobulin; monoclonal antibody; against I-A b, I-A d, I-A q, I-E d and I-E k
Comments	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵ viable cells/mL. Maintain cell density between 5 x 10⁴ and 5 x 10⁵ viable cells/mL. Medium Renewal: Every 2 to 4 days
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: 5% CO₂
Isotype	rat IgG2b kappa
Name of Depositor	TA Springer
Deposited As	rat (B cell); mouse (myeloma)
References	Bhattacharya A, et al. A shared alloantigenic determinant on 1a antigens encoded by the I-A and I-E subregions: evidence for 1 region gene duplication. J. Immunol. 127: 2488-2495, 1981. PubMed: 6170707 Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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