| 產品名稱 |
MA1-6 |
| 商品貨號 |
B165059 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody also binds to the P. fluorescens group of the Family Pseudomonadaceae and with Azotobacter vinelandii, but not with other gram negative bacteria. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified outer membranes of P. aeruginosa PAO1 strain H1O3. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the lipoprotein H2 of Pseudomonas aeruginosa |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the lipoprotein H2 of Pseudomonas aeruginosa |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with purified outer membranes of P. aeruginosa PAO1 strain H1O3. Spleen cells were fused with NS-1 myeloma cells. The antibody reacted with lipoprotein H2 on 50 of the 52 strains of P. aeruginosa that were tested. The antibody also binds to the P. fluorescens group of the Family Pseudomonadaceae and with Azotobacter vinelandii, but not with other gram negative bacteria. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
RW Hancock |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hancock RE, et al. Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens: isolation and characterization. Infect. Immun. 37: 166-171, 1982. PubMed: 6809625
Mutharia LM, Hancock RE. Monoclonal antibody for an outer membrane lipoprotein of the Pseudomonas fluorescens Group of the Family Pseudomonadaceae. Int. J. Syst. Bacteriol. 35: 530-532, 1985.
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