| 產品名稱 |
mAB 24-1 [mAb 24-1] |
| 商品貨號 |
B165064 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody binds an epitope comprising amino acids 1477-1480 of human CFTR. The antibody can be used to purify CFTR from an impure solution containing CFTR, to detect CFTR in a biological sample, and to determine the orientation of CFTR in the membrane of a lipid vesicle. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with myeloma P3X63Ag8 cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the cystic fibrosis transmembrane conductance regulator (CFTR) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the cystic fibrosis transmembrane conductance regulator (CFTR) |
| Comments |
Animals were immunized with a fusion protein comprising glutathione S-transferase and amino acids 1377-1480 of the human cystic fibrosis transmembrane conductance regulator (CFTR). Spleen cells were fused with myeloma P3X63Ag8 cells. The antibody binds an epitope comprising amino acids 1477-1480 of human CFTR. The antibody can be used to purify CFTR from an impure solution containing CFTR, to detect CFTR in a biological sample, and to determine the orientation of CFTR in the membrane of a lipid vesicle. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium, 80%; additional fetal bovine serum, 10%; DMSO, 10% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
Genzyme Corp. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Cheng SH, et al. Antibodies specific for cystic fibrosis transmembrane conductance regulator and uses therefor. US Patent 5,981,714 dated Nov 9 1999
|
