| 產品名稱 |
MAb 5.2 [#5.2] |
| 商品貨號 |
B165068 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 185000 dalton polypeptide (and processing fragments of the polypeptide) from P. falciparum merozoites. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacted with 7 isolates of P. falciparum obtained from 7 different geographical areas. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Plasmodium falciparum merozoite antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Plasmodium falciparum merozoite antigen |
| Comments |
Animals were immunized with schizont enriched P. falciparum. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a 185000 dalton polypeptide (and processing fragments of the polypeptide) from P. falciparum merozoites. The antibody reacted with 7 isolates of P. falciparum obtained from 7 different geographical areas. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose with 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
fetal bovine serum, 90%; DMSO, 10% |
| Isotype |
IgG2b |
| Name of Depositor |
University of Hawaii |
| U.S. Patent Number |
|
| References |
Siddiqui WA. Monoclonal antibody-specific merozoite antigens. US Patent 4,897,354 dated Jan 30 1990
|
