| 產品名稱 |
mAb35 |
| 商品貨號 |
B165072 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to the major immunogenic region of the alpha subunits, and cross-reacts well with acetylcholine receptors of a variety of species. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized subcutaneously with purified, intact Electrophorus electricus acetylcholine receptor. Spleen cells were fused with S194 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against acetylcholine receptor (alpha subunit) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against acetylcholine receptor (alpha subunit) |
| Comments |
Animals were immunized subcutaneously with purified, intact Electrophorus electricus acetylcholine receptor. Spleen cells were fused with S194 myeloma cells. The antibody binds to the major immunogenic region of the alpha subunits, and cross-reacts well with acetylcholine receptors of a variety of species. It can passively transfer experimental autoimmune myasthenia gravis. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's Modified Eagle's Medium, 4.5 g/L glucose with 0.1 mM non-essential amino acids, 90%; fetal bovine serum, 10% |
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/ml and maintain between 1 x 105 and 1 x 106 cells/ml.
Medium Renewal: Every 2 to 3 days |
| Isotype |
IgG1 |
| Antigenic Determinant |
Acetylcholine receptor (AChR) alpha subunit |
| Name of Depositor |
J Lindstrom |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Tzartos SJ, et al. Mapping of surface structures of Electrophorus acetylcholine receptor using monoclonal antibodies. J. Biol. Chem. 256: 8635-8645, 1981. PubMed: 6167581
Tzartos SJ, et al. Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 79: 188-192, 1982. PubMed: 6948300
Sargent PB, et al. Structure and transmembrane nature of the acetylcholine receptor in amphibian skeletal muscle as revealed by cross-reacting monoclonal antibodies. J. Cell Biol. 98: 609-618, 1984. PubMed: 6363425
Swanson LW, et al. Immunohistochemical localization of monoclonal antibodies to the nicotinic acetylcholine receptor in chick midbrain. Proc. Natl. Acad. Sci. USA 80: 4532-4536, 1983. PubMed: 6192437
|
