| 產品名稱 |
mAb64 |
| 商品貨號 |
B165073 |
| Organism |
Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? Lou (B cell); Lou (myeloma) |
| Applications |
The antibody is specific for the alpha subunit of AChR and does not react with the main immunogenic region of AChR. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized subcutaneously with purified, intact bovine calf acetylcholine receptor. Spleen cells were fused with Y3 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against acetylcholine receptor (AChR) alpha subunit |
| Cellular Products |
immunoglobulin; monoclonal antibody; against acetylcholine receptor (AChR) alpha subunit |
| Comments |
Animals were immunized subcutaneously with purified, intact bovine calf acetylcholine receptor. Spleen cells were fused with Y3 myeloma cells. The antibody is specific for the alpha subunit of AChR and does not react with the main immunogenic region of AChR. The antibody a has low affinity for S. aureus protein A. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.01 mg/ml insulin, 0.01 mg/ml transferrin, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
Salk Institute for Biological Studies |
| Deposited As |
rat (B cell); rat (myeloma) |
| U.S. Patent Number |
|
| References |
Lindstrom JM. Assays for myasthenia gravis. US Patent 4,789,640 dated Dec 6 1988
Tzartos S, et al. Characteristics of monoclonal antibodies to denatured Torpedo and to native calf acetylcholine receptors: species, subunit and region specificity. J. Neuroimmunol. 10: 235-253, 1986. PubMed: 3484485
|
