| 產品名稱 |
MB40.5 |
| 商品貨號 |
B165092 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a monomorphic HLA A, B, C specificity and cross-reacts with various primate species. Tested and found negative for ectromelia virus (mousepox). NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Comments |
Animals were immunized with papain solubilized HLA B40 antigen. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a monomorphic HLA A, B, C specificity and cross-reacts with various primate species. The antibody is not cytotoxic. Tested and found negative for ectromelia virus (mousepox). NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state. |
| Complete Growth Medium |
Modified Dulbecco's medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Parham P. Monoclonal antibodies against two separate alloantigenic sites of HLA-B40. Immunogenetics 13: 509-527, 1981. PubMed: 7026428
|
