| 產品名稱 |
MC116 |
| 商品貨號 |
B165099 |
| Organism |
Homo sapiens, human |
| Tissue |
ascites |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
undifferentiated lymphoma |
| Gender |
male |
| Karyotype |
45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23) |
| Clinical Data |
male |
| Antigen Expression |
HLA A1, A3, B8, Bw35 |
| Genes Expressed |
immunoglobulin (surface and secreted) |
| Cellular Products |
immunoglobulin (surface and secreted) |
| Comments |
The cells are EBNA negative, negative for Fc receptors and negative for complement receptors. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 105 viable cells/mL and maintain at or below 3 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Isotype |
IgM; lambda light chain |
| Population Doubling Time |
26 to 28 hrs |
| Name of Depositor |
IT Magrath |
| Deposited As |
Homo sapiens |
| References |
Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721
Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722
|
| Cross References |
Nucleotide (GenBank) :
U07991
Human lymphoma immunoglobulin lambda light chain mRNA, partial cds.
Nucleotide (GenBank) :
U07988
Human lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds.
|
