| 產品名稱 |
MK-D6 |
| 商品貨號 |
B165161 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-A d; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-A d; cytotoxic |
| Comments |
Mice were immunized with spleen cells from B10.D2 mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not react with I-Ak, I-Ab, I-As, I-Af or I-Aa. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
J Kappler, P Marrack |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kappler JW, et al. Antigen-inducible, H-2 restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J. Exp. Med. 153: 1198-1214, 1981. PubMed: 6166712
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