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MPC 11 OUAr

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編號:B165188

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產品名稱	MPC 11 OUAr
商品貨號	B165188
Organism	Mus musculus, mouse
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	plasmacytoma; myeloma
Strain	BALB/c
Applications	This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). Tested and found negative for ectromelia virus (mousepox).
Derivation	This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse).
Antigen Expression	H-2d
Genes Expressed	immunoglobulin; monoclonal antibody,H-2d
Cellular Products	immunoglobulin; monoclonal antibody
Comments	This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype	IgG2b
Name of Depositor	M Cohn
Deposited As	Mus musculus
References	Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836 Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146 Gefter ML, et al. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet. 3: 231-236, 1977. PubMed: 605383 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

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