| 產品名稱 |
MYC 1-9E10.2 [9E10] |
| 商品貨號 |
B165213 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
Spleen |
| Cell Type |
Hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
Lymphoblast-like |
| Culture Properties |
Suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc. |
| Derivation |
Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human myc (c-myc) protein |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human myc (c-myc) protein |
| Comments |
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Isotype |
IgG1 |
| Name of Depositor |
J Bishop, GI Evan |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782
Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077
Cheng M, et al. ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539
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