波多野结衣毛片-乳色吐息在线观看-国产伦子伦对白视频-性做久久久-狠狠干2019-黄色裸体片-美女无遮挡免费网站-国产91熟女高潮一区二区-懂色av蜜臀av粉嫩av分享-小h片在线观看-台湾佬在线-日韩激情在线播放-欧日韩不卡在线视频-波多野结衣中文字幕一区-天天操夜夜草

• 0.005 mg/ml Insulin (Sigma I1882)
• 0.01 mg/ml Transferrin (Sigma T1147)
• 30nM Sodium selenite (final conc.) (Sigma S9133) 
• 10 nM Hydrocortisone (final conc.) (Sigma H0135)
• 10 nM beta-estradiol (final conc.) (Sigma E2557)  
• extra 2mM L-glutamine (for final conc. of 4.5 mM) (Sigma G5783) 
• 5% fetal bovine serum (ATCC 30-2020)

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.