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編號:B165260

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產(chǎn)品名稱	NCI-H1385 [H1385]
商品貨號	B165260
Organism	Homo sapiens, human
Tissue	lung; derived from metastatic site: lymph node
Product Format	frozen
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage 3A, carcinoma; non-small cell lung cancer
Age	49 years
Gender	female
Ethnicity	Black
Storage Conditions	liquid nitrogen vapor phase
Derivation	The line was established in April 1986 from lymph node, a metastatic site.
Clinical Data	49 years old female Black The patient was a smoker, 33 packs year.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. Medium Renewal: 2 times per week
Cryopreservation	Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 13 D7S820: 8,10 THO1: 7 TPOX: 8,9 vWA: 17,19
Name of Depositor	AF Gazdar, JD Minna
Deposited As	Homo sapiens
Year of Origin	1986
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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