| 產品名稱 | NCI-H23 [H23] |
|---|---|
| 商品貨號 | B165331 |
| Organism | Homo sapiens, human |
| Tissue | lung |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma; non-small cell lung cancer |
| Age | 51 years |
| Gender | male |
| Ethnicity | Black |
| Images |  |
| Derivation | This line was derived from a lung cancer obtained from a patient prior to therapy.
|
| Clinical Data | 51 years Black male This line was derived from a lung cancer obtained from a patient prior to therapy. |
| Oncogene | myc +; src +; abl +; erb +; ras +; sis - |
| Genes Expressed | myc +; src +; abl +; erb +; ras +; sis - |
| Comments | The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene.
There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs.
The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).
NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA.
The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.
NCI-H23 cells are L-dopa decarboxylase-negative.
They have a reported colony forming efficiency of 9.7% in soft agarose.
|
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature. Remove the trypsin and incubate the flask at 37°C for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Culture medium, 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 THO1: 6 TPOX: 8,9 vWA: 16,17 |
| Population Doubling Time | 38 hrs |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| References | Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201 Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Mitsudomi T, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7: 171-180, 1992. PubMed: 1311061 Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086 Forsberg K, et al. Expression of functional PDGF beta receptors in a human large-cell lung- carcinoma cell line. Int. J. Cancer 53: 556-560, 1993. PubMed: 8382192 Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Lung Cancer 4: 155-161, 1988. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
