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NCI-H2405 [H2405]

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編號:B165335

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產(chǎn)品名稱	NCI-H2405 [H2405]
商品貨號	B165335
Organism	Homo sapiens, human
Tissue	lung
Product Format	frozen
Culture Properties	mixed, adherent and suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage 4, adenocarcinoma; non-small cell lung cancer
Age	47 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase
Derivation	The line was established in September 1990 from ascites, a metastatic site.
Clinical Data	The patient was a smoker. 75 pack years.
Complete Growth Medium	The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cells and as attached epithelial-like cells. Attached cells may be subcultured using 0.25% trypsin - 0.53mM EDTA. Subcultivation Ratio: 1:5 to 1:8 is recommended. Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation	85% Culture Media + 10% FBS+ 5% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor	AF Gazdar, JD Minna
Deposited As	Homo sapiens
Year of Origin	1990
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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