| 產品名稱 |
NCI-H345 [H345] |
| 商品貨號 |
B165341 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: bone marrow |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
suspension, multicell aggregates and some adherent cells
(This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.) |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
carcinoma; small cell lung cancer |
| Age |
64 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
hypotriploid; modal number = 63; range = 60 to 66.
Sixteen or more marker chromosomes were common to most cells. Nearly half of the markers had lengths close to the D group or smaller chromosomes. Among the identifiable markers were der(1)t(1;17)(q21;q11), der(?9)t(1;?9)()q12;?q21, t(8q?12q), t(12q?17q), der(9)t(9,?)(q22;?). Normal N13 was absent; the N8, N9, N12 and N19 were found as single copies; N20 generally had 4 copies; there were 2 normal X chromosomes and no Y chromosome was detected. |
| Derivation |
The NCI-H345 cell line was derived by D. Carney, A.F. Gazdar and associates in 1981 from the bone marrow of a patient with small cell carcinoma of the lung (SCLC). The tumor specimen was obtained after the initiation of chemotherapy. |
| Clinical Data |
64 years
Caucasian
male |
| Cellular Products |
gastrin releasing peptide |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice (The cells form transplantable tumors with typical small cell carcinoma histology.) |
| Comments |
The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.
The line does not exhibit any gross structural DNA abnormalities.
The cells express elevated levels of four biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.
The cells form transplantable tumors with typical small cell carcinoma histology.
|
| Complete Growth Medium |
HITES medium (serum free) is formulated at the ATCC as follows:
- DMEM: F-12 Medium (ATCC 30-2006)
-
Insulin 0.005mg/ml (Gibco 12585-014)
-
Transferrin 0.01 mg/ml (Sigma T5391 or equivalent)
-
Sodium selenite 30 nM (Sigma S9133 or equivalent)
-
Hydrocortisone 10 nM (Sigma H0135 or equivalent)
-
beta-estradiol 10 nM (Sigma E2257 or equivalent)
-
L-Glutamine Solution (ATCC 30-2214) 2 mM (in addition to that in the base medium)
|
| Subculturing |
Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. Aggregates may be dispersed by trituration. The use of trypsin is not recommended.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 9,11 D16S539: 8 D5S818: 11,12 D7S820: 9,13 THO1: 9,9.3 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 0 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1981 |
| References |
Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893
This cell line grows as gland-like clusters in suspension with a few attached cells. The viability of the cells in clusters is better than the viability of the single cells in the cultures.
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