| 產(chǎn)品名稱 |
NCI-H508 [H508] |
| 商品貨號 |
B165347 |
| Organism |
Homo sapiens, human |
| Tissue |
cecum |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
floating aggregates of round cells with some attached cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
colorectal adenocarcinoma |
| Age |
55 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Karyotype |
modal number = 102; range = 71 to 131; most cells have DM's |
| Derivation |
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. |
| Clinical Data |
55 years Caucasian male |
| Antigen Expression |
Blood type A; Rh+; CA19-9 antigen |
| Genes Expressed |
carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days |
| Cellular Products |
carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells
|
| Comments |
The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove culture medium. Keep floating cells.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C.to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension and the harvested cells to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 12 D7S820: 8,12 THO1: 9,9.3 TPOX: 8,11 vWA: 15,16 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor |
AF Gazdar |
| Deposited As |
Homo sapiens |
| References |
Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
|
