| 產(chǎn)品名稱 |
NCI-H660 |
| 商品貨號(hào) |
B165356 |
| Organism |
Homo sapiens, human |
| Tissue |
prostate; derived from the metastatic site: lymph node |
| Cell Type |
epithelial neuroendocrine |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
floating and attached clusters |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage E, small cell carcinoma |
| Age |
63 years adult |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Derived from metastatic site, lymph node |
| Clinical Data |
63 years adult Caucasian male |
| Receptor Expression |
atrial natriuretic peptide (ANP), expressed |
| HeLa Markers |
N |
| Comments |
While NCI-H660 has the appearance and many of the properties of small cell carcinoma, there are also differences. NCI-H660 expresses elevated levels of L-dopa carboxylase and bombesin-like immunoreactivity. The cells express functional atrial natriuretic peptide (ANP) receptors, but ANP addition produces no detectable change in the cells' growth pattern. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No.30-2001. To make the complete growth medium, add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Add medium as the cell density increases. Shake flask to dislodge attached cells. Single cells are often dead and clusters are viable. Subculture when there are numerous, healthy-appearing clusters present in suspension.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 40%; fetal bovine serum, 50%; DMSO, 10%.
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 8 D16S539: 11 D5S818: 11 D7S820: 8,10 THO1: 7,9.3 TPOX: 8 vWA: 17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| References |
Lai SL, et al. Molecular genetic characterization of neuroendocrine lung cancer cell lines. Anticancer Res. 15: 225-232, 1995. PubMed: 7762988
Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
Johnson BE, et al. Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies. J. Natl. Cancer Inst. 81: 1223-1228, 1989. PubMed: 2569043
NCI-H660 was originally described as a small cell lung carcinoma [PubMed: 2985257], subsequently it was correctly identified as an extrapulmonary small cell carcinoma originating in the prostate gland [PubMed: 2569043, 7762988]. The line was derived by A. F. Gazdar, H. K. Oie, J. D. Minna and associates from a lymph node metastasis taken from a patient prior to therapy.
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