| 產品名稱 |
NCI-H841 [H841] |
| 商品貨號 |
B165373 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Culture Properties |
mixed: adherent and suspension, Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage L,carcinoma; variant small cell lung cancer |
| Age |
51 years |
| Gender |
male |
| Ethnicity |
Caucasian, White |
| Applications |
The line was established in October 1984. |
| Karyotype |
t(2p,3p), t(3q,9q) |
| Derivation |
The line was established in October 1984. |
| Clinical Data |
The patient received prior chemotherapy and radiation therapy. The patient was a smoker. male Caucasian, White 51 years |
| Comments |
The line was established in October 1984. The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 70. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Add fresh medium as cell density increases. Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11 D13S317: 12 D16S539: 13 D5S818: 11,12 D7S820: 10,13 THO1: 6,9 TPOX: 11,12 vWA: 16,17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1984 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
