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NCTC 3749
NCTC 3749
規格:
貨期:
編號:B165380
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 NCTC 3749
商品貨號 B165380
Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain DBA/2
Applications
When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of P388D1 (ATCC CCL-46).
Antigen Expression
H-2d
Genes Expressed
produce interleukin-1 (IL-1, interleukin 1),H-2d
Cellular Products
produce interleukin-1 (IL-1, interleukin 1)
Virus Resistance
poliovirus 1
Comments
This line is a derivative of P388D1 (ATCC CCL-46).
When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium NCTC 135 medium, 100%
Subculturing

Subcultures are prepared by scraping. Remove one-half of the old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.

Subcultivation Ratio: A ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2)
Name of Depositor VJ Evans
Deposited As Mus musculus
References

Mizel SB, et al. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497-1503, 1978. PubMed: 149171

Evans VJ, et al. A new tissue culture isolation and explanation of the P388 lymphocytic neoplasm in a chemically characterized medium. Exp. Cell Res. 32: 212-217, 1963. PubMed: 14094040

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957

Rabson AS, Leggallais FY. Cytopathogenic effect produced by polyoma virus in cultures of milk-adapted murine lymphoma cells (strain P388 D1). Proc. Soc. Exp. Biol. Med. 100: 229-233, 1959. PubMed: 13634094

. . Nature 181: 1343, 1958.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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李經理 13626845108 972239479
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