| 產品名稱 |
NRK-52E |
| 商品貨號 |
B165412 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Receptor Expression |
epidermal growth factor (EGF); multiplication stimulating activity (MSA) |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.02% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh culture medium, aspirate and dispense into new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
JE DeLarco |
| Deposited As |
Rattus sp. |
| References |
de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742
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