| 產品名稱 |
OKT 4 |
| 商品貨號 |
B165433 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
Spleen, blood |
| Cell Type |
Hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
Lymphoblast-like |
| Culture Properties |
Suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
CAF1 (B cell); BALB/c (myeloma) |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Mice were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with P3X63Ag8.U1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human T cell (human helper/inducer T lymphocyte); against CD4 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human T cell (human helper/inducer T lymphocyte); against CD4 |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20% |
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO.
Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C |
| Isotype |
IgG2b |
| Name of Depositor |
Ortho Pharmaceutical Corporation |
| U.S. Patent Number |
|
| References |
Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909
Kung PC, Goldstein G. Monoclonal antibody to human helper T cells and methods of preparing same. US Patent 4,381,295 dated Apr 26 1983
Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218
Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998
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