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OKT 8

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編號:B165437

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產品名稱	OKT 8
商品貨號	B165437
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Animals were immunized with human peripheral blood T lymphocytes. Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte); against CD8
Cellular Products	immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte); against CD8
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cultures at a cell concentration between 2 x 10⁵ and 1 x 10⁶cells/mL. Medium Renewal: Every 2 to 3 days
Culture Conditions	Temperature: 37°C
Isotype	IgG2a
Name of Depositor	Ortho Pharmaceutical Corporation
U.S. Patent Number	4,361,550
References	Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909 Kung PC, Goldstein G. Complement-fixing monoclonal antibody to human suppressor T cells and methods of preparing same. US Patent 4,361,550 dated Nov 30 1982 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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