| 產(chǎn)品名稱 |
P116.cl39 [P116.c39] |
| 商品貨號(hào) |
B165449 |
| Organism |
Homo sapiens, human |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, multicell aggregates |
| Biosafety Level |
2 [Cells contain CMV and SV-40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute T cell leukemia |
| Gender |
male |
| Applications |
The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP. P116.cl39 cells stably expresses the ZAP-70 kinase. The cell line can be used as a control for the ZAP-70 negative P116 cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP. |
| Clinical Data |
male |
| Comments |
The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP. The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. P116.cl39 cells stably expresses the ZAP-70 kinase. The cell line can be used as a control for the ZAP-70 negative P116 cells. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 2 to 3 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
RT Abraham |
| Deposited As |
human |
| References |
Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454
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