| 產品名稱 |
P3X63Ag8U.1 |
| 商品貨號 |
B165459 |
| Organism |
Mus musculus, mouse |
| Cell Type |
B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
plasmacytoma; myeloma |
| Gender |
female |
| Strain |
BALB/c |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was derived from the MOPC 21 - P3X63Ag8 lineage. |
| Clinical Data |
female |
| Antigen Expression |
H-2d |
| Comments |
The cells do not synthesize gamma 1 chains.
They produce but do not secrete kappa chains.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 x 105 viable cells/mL and maintain below 1 x 106 cells/mL.
Medium Renewal: 3 times per week |
| Cryopreservation |
Freeze medium: Culture medium, 95%; DMSO, 5%. |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
MD Scharff |
| Deposited As |
Mus musculus |
| References |
Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551
|
| Cross References |
Nucleotide (GenBank) :
E02974
DNA encoding mouse interleukin-6(IL-6) receptor.
|
