| 產品名稱 |
PA2.1 |
| 商品貨號 |
B165465 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reactive correlates with the classical HLA A2 specificity. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified HLA A2. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA A2, Aw69 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA A2, Aw69 |
| Comments |
Animals were immunized with purified HLA A2. Spleen cells were fused with NS-1 myeloma cells. Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in complete loss of binding of PA2.1. The antibody reactive correlates with the classical HLA A2 specificity. PA2.1 and BB7.2 (ATCC HB-82) recognize overlapping epitopes on the alpha 2 domain of HLA-A2. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Parham P, Bodmer WF. Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2. Nature 276: 397-399, 1978. PubMed: 714164
Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229
Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666
Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015
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