Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with a denaturation sensitive determinant on the large T antigen.
It reacts with higher affinity than PAb 100 (ATCC TIB-115) but does recognized a subclass of large T antigen recognized by sera from mice bearing SV40 induced tumors.
Reacts strongly by immunofluorescence with nuclei of SV40 transformed cells but not with BK virus infected human cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against large T antigen (SV40)
Cellular Products
immunoglobulin; monoclonal antibody; against large T antigen (SV40)
Comments
Animals were immunized with the SV40 transformed 3T3 clone 4 mouse cell line (B4).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a denaturation sensitive determinant on the large T antigen.
It reacts with higher affinity than PAb 100 (ATCC TIB-115) but does recognized a subclass of large T antigen recognized by sera from mice bearing SV40 induced tumors.
Reacts strongly by immunofluorescence with nuclei of SV40 transformed cells but not with BK virus infected human cells.
The antibody precipitates large T antigen and NVT (when complexed) but not small t antigen (nor the smaller SV-80 t antigen) from extracts of SV-80 cells and SV40 infected TC-7 cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp4 cells/ml and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
Isotype
IgG2a
Name of Depositor
EG Gurney
Deposited As
mouse (B cell); mouse (myeloma)
References
Gurney EG, et al. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct subclasses of large T antigen and for similarities among nonviral T antigens. J. Virol. 34: 752-763, 1980. PubMed: 6155477
Deppert W, et al. Monoclonal antibodies against simian virus 40 tumor antigens: analysis of antigenic binding sites, using adenovirus type 2-simian virus 40 hybrid viruses. J. Virol. 37: 478-482, 1981. PubMed: 6163870
