| 產品名稱 |
PAb 108 |
| 商品貨號 |
B165478 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody cross-reacts with the large T antigen of BK virus. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40) |
| Comments |
Animals were immunized with SV40 transformed BALB/3T3 cells. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with the large T antigen of BK virus. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, 80%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
EG Gurney |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584
|
