Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with a denaturation stable determinant on the 53000 dalton mouse NVT antigen.
Also binds to similar (51000 to 56000 dalton) NVT antigens SV40 transformed or lytically infected mouse, rat, hamster, monkey and human cells as well as some uninfected mouse cell lines.
The antibody binds to denatured mouse NVT antigen but not to large T antigen and by immunofluorescence reacts moderately to strongly with SV40 infected cells and BK virus infected human cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse non-viral T antigen (NVT)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse non-viral T antigen (NVT)
Comments
Animals were immunized with the SV40 transformed 3T3 clone 4 mouse cell line (B4).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a denaturation stable determinant on the 53000 dalton mouse NVT antigen.
Also binds to similar (51000 to 56000 dalton) NVT antigens SV40 transformed or lytically infected mouse, rat, hamster, monkey and human cells as well as some uninfected mouse cell lines.
The antibody binds to denatured mouse NVT antigen but not to large T antigen and by immunofluorescence reacts moderately to strongly with SV40 infected cells and BK virus infected human cells.
Weak immunofluorescence activity is seen with BALB/3T12 cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
EG Gurney
Deposited As
mouse (B cell); mouse (myeloma)
References
Gurney EG, et al. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct subclasses of large T antigen and for similarities among nonviral T antigens. J. Virol. 34: 752-763, 1980. PubMed: 6155477
Deppert W, et al. Monoclonal antibodies against simian virus 40 tumor antigens: analysis of antigenic binding sites, using adenovirus type 2-simian virus 40 hybrid viruses. J. Virol. 37: 478-482, 1981. PubMed: 6163870
Crawford LV, et al. Detection of a common feature in several human tumor cell lines -- a 53,000 dalton protein. Proc. Natl. Acad. Sci. USA 78: 41-45, 1981. PubMed: 6264441
