| 產(chǎn)品名稱 |
PDL-1 |
| 商品貨號(hào) |
B165491 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the cell surface of periodontal ligament cells of bovine and baboon origin. The antibody also reacts with cells of mesenchymal origin from a number of organs. |
| Derivation |
Spleen cells were fused with FOX-NY myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a cell surface antigen on bovine periodontal ligament cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a cell surface antigen on bovine periodontal ligament cells |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with cultured bovine periodontal ligament cells. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with the cell surface of periodontal ligament cells of bovine and baboon origin. The antibody also reacts with cells of mesenchymal origin from a number of organs. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
RJ Klebe |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
DuBois WT, et al. Monoclonal antibodies to periodontal ligament cells. J. Periodontol. 62: 190-196, 1991. PubMed: 2027070
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