| 產(chǎn)品名稱 |
PG-4 (S+L-) |
| 商品貨號 |
B165501 |
| Organism |
Felis catus, cat |
| Tissue |
brain |
| Cell Type |
astrocyte; Moloney murine sarcoma virus (Mo-MSV) transformed |
| Product Format |
frozen |
| Morphology |
glial, astrocyte |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
embryo |
| Applications |
This cell line is useful for detection and quantitation of replication competent retroviruses. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Virus Resistance |
murine leukemia virus (MuLV) |
| Comments |
The line is resistant to infection and focus formation by ecotropic MuLV. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
heat-inactivated fetal bovine serum to a final concentration of 10%
|
| Subculturing |
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin, add fresh trypsin (1 to 2 mL) and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
KJ Dunn |
| Deposited As |
Felis catus |
| Year of Origin |
May, 1980 |
| References |
Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093
Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413
Li Z, et al. PG-4 cell plaque assay for xenotropic murine leukemia virus. J. Virol. Methods 81: 47-53, 1999. PubMed: 10488760
The cells are useful for detection and assay of various C type retroviruses. PG-4 is one of the cell lines that is recommended by the U.S. Food and Drug Administration for detection of replication competent retroviruses in products and reagents for human use.
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